SORBITOL MACCONKEY AGAR (SMAC AGAR) RENDIMENTO 51,5g/l MERCK EMB 500 GR

SORBITOL MACCONKEY AGAR (SMAC AGAR) MERCK RENDIMENTO 51,5g/l MS 80141300389 EMBALAGEM 500G Norm 10167 for the detection of Escherichia coli (E. coli) serotype 0157:H7 in foods as well as with the methods of FDABAM for the isolation of enterohemorrhagic E. coli (EHEC). Mode of Action Bile salts mixture and crystal violet largely inhibit the growth of the Gram-positive microbial flora. The addition of Cefixime Potassium tellurite (CT) Supplement increases the selectivity for E. coli 0157:H7 and suppresses the remaining accompanying flora. For the detection of E. coli 0157:H7 the CT-SMAC Agar method is superior to the HC Agar (SZABO) method according to the study of WEAGANT (1995). Sorbitol, together with the pH indicator neutral red, is used to detect sorbitol-positive colonies and turning them red in colour. Sorbitol-negative strains, on the other hand, form colourless colonies. Typical Composition (g/litre) Peptone 20.0; sodium chloride 5.0; bile salts No. 3 1.5; sorbitol10.0; crystal violet 0.001; neutral red 0.03; agar-agar 15.0.