ROSE BENGAL CHLORANFENICOL (RBC) AGAR MERCK - EMB 500G

ROSE BENGAL CHLORANFENICOL (RBC) AGAR MERCK Embalagem 500g Mode of Action The neutral pH in combination with chloramphenicol suppresses the growth of most bacteria. Rose bengal, taken up intracellular by fungi, restricts the size and the spreading of moulds, preventing overgrowth of slow growing species by luxuriant species. Typical Composition (g/litre) Mycological peptone 5.0; glucose 10.0; potassium dihydrogen phosphate 1.0; magnesium sulfate 0.5; Rose Bengal 0.05; chloramphenicol 0.1; agar-agar 15.5 pH 7.2 ± 0.2 at 25 °C. Preparation Suspend 32.2 g in 1 liter of demin. water and heat to boiling until completely dissolved. Autoclave the medium at 121 °C for 15min. Cool to approx. 50 °C, mix well and pour plates. The appearance of the prepared medium is pink to red . When stored at +2 to +8 °C in the dark, the shelf life of plates is approximately 1 week and in bottles approx. 2 months.