REINFORCED CLOSTRIDIUM MEDIUM (RCM) AGAR MERCK - EMB 500G

REINFORCED CLOSTRIDIUM MEDIUM (RCM) AGAR RENDIMENTO 50,0g/l Embalagem 500g Mode of Action This culture medium is free from inhibitors and contains cysteine as a reducing agent. According to HIRSCH and GRINSTED (1954), Polymyxin B can be added to inhibit Gram-negative bacteria. Typical Composition (g/litre) Meat extract 10.0; peptone from casein 10.0; yeast extract 3.0; D(+)glucose 5.0; starch 1.0; sodium chloride 5.0; odium acetate 3.0; L-cysteinium chloride 0.5; agar-agar 12.5. Preparation Suspend 50 g/litre, if desired dispense into test tubes, autoclave (15 min at 121 °C). If required, cool to 45-50 °C and add 0.02 g Polymyxin B/litre in form of a filter-sterilized aqueous solution. pH: 6.8 ± 0.2 at 25 °C. The medium in the tubes or Petridishes is clear and yellowishbrown.