REINFORCED CLOSTRIDIUM MEDIUM (RCM) SEG HARM EP/USP/JP MERCK - EMB 500G

REINFORCED CLOSTRIDIUM MEDIUM (RCM) SEG HARM EP/USP/JP RENDIMENTO 33,0g/l Embalagem 500g Medium proposed by HIRSCH and GRINSTED (1954) for the cultivation and enumeration of clostridia, other anaerobes and facultative microorganisms in foodstuffs, clinical specimens and other materials. Mode of Action See Reinforced Clostridial Agar. Typical Composition (g/litre) Meat extract 10.0; peptone 5.0; yeast extract 3.0; D(+)glucose 5.0; starch 1.0; sodium chloride 5.0; sodium acetate 3.0; L-cysteinium chloride 0.5; agar-agar 0.5 Preparation Dissolve 33 g/litre, dispense into test tubes, autoclave (15 min at 121 °C). Cool, if required add 0.02 g Polymyxin B/litre in form of an aqueous solution and mix. pH: 6.8 ± 0.2 at 25 °C. The prepared medium in the tubes is clear and yellowish.