MSRV MEIO BASE MODIFICADO MERCK - EMB 500G

MSRV MEIO BASE MODIFICADO MERCK RENDIMENTO 31,6g/l MODIFIED SEMI-SOLID RAPPAPORT-VASSILIADIS EMBALAGEM 500G MSRV Medium is a semi-solid medium used for the isolation of Salmonella from food-stuffs and other materials. Modified Semi-solid RappaporMt-SVRaVss Milieaddiius m(M BSaRsVe), Mmoeddiiufim. Mode of Action De SMEDT et al. (1986) made a semi-solid RV-medium by adding agar (MSRV). In comparison to traditional methods this formulation gave more Salmonella-positive results. The detection principle is based on the motility of Salmonellae to migrate into the semi-solid medium thus forming opaque halos of growth. The motility of other microorganisms is largely inhibited by selective agents (Magnesium chloride, Malachite green and Novobiocin) and the enhanced incubation temperature of 42 °C. Typical Composition (g/litre) Tryptose 4.59; casein hydrolysate 4.59; sodium chloride 7.34; potassium dihydrogen phosphate 1.47; Magnesium chloride anhydrous 10.93; malachite green 0.037; agar-agar 2.7.