MEIO DE CULTURA DE TESTE OF BASAL MEDIUM BASE SEG HUGH & LEIFSON RENDIMENTO 11,0g/l MERCK EMB 500GR

OF BASAL MEDIUM BASE SEG HUGH & LEIFSON MERCK RENDIMENTO 11,0g/l EMBALAGEM 500G Test culture medium proposed by HUGH and LEIFSON (1953) for deltecting oxidative and fermentative carbohydrate degradation. It is used primarily for the differentiation and classification of gram-negative intestinal bacteria. A selective and differential agar for Pseudomonas cepacia was conceived by WELCH et al. (1987) on the basis of this medium, with the addition of agar-agar, lactose, polymyxin B and bacitracin. Principle Microbiological method Mode of Action A carbohydrate is added to the culture medium, degradation of the carbohydrate to acid is indicated by the pH indicator bromothymol blue which changes its colour to yellow. The degradation is allowed to take place while the medium is exposed to air (degradation may be oxidative or fermentative) or under exclusion of air (degradation by fermentation only). Typical Composition (g/litre) Peptone from casein 2.0; yeast extract 1.0; sodium chloride 5.0; di-potassium hydrogen phosphate 0.2; bromothymol blue 0.08; agar-agar 2.5. also to be added: carbohydrate 10.0 g/l.