MALACHITE GREEN BROTH BASE 500GR MERCK

MALACHITE GREEN BROTH BASE MERCK RENDIMENTO 8,4 (SIMPLES) OU 25,1g/l (TRIPLO) EMBALAGEM 500GR Typical Composition (g/litre) Peptone from meat 5.0; meat extract 3.0; di-potassium hydrogen phosphate 0.37. These values are valid for the single-strength broth. Preparation Base Broth: Suspend 8.4 g (single-strength broth) or 25.1 g (triple-strength broth) in 1 litre of demineralized water. Dispense in 50 ml volumes into suitable culture vessels and autoclave (15 min at 121°C). The broth base is clear and yellow-brown. Malachite-green solution: Suspend 0.15 g malachite-green oxalate in 90 ml of deminera-lized water and sterilize by filtration. Complete broth: Add to 50 ml of cooled base broth 0.3 ml (single-strength broth) or 0.9 ml (triple-strength broth) of the malachite-green solution under sterile conditions. pH: 7.3 ± 0.2 at 25 °C. The complete broth is clear and green. Alternative method of preparation: If preferred, the malachite-green solution may also be added before distributing the base broth into the vessels. In this case it has to be autoclaved first. Then add to 1 litre of base broth 6ml (single-strength broth) or 18 ml (triple-strength broth) of malachite-green solution under sterile conditions and dispense in 50 ml volumes into sterile vessels.