M TSB BROTH COM NOVOBIOCINA MERCK - EMB 50G

M TSB BROTH COM NOVOBIOCINA MERCK RENDIMENTO 33,0g/l EMBALAGEM 500G For the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods. The culture medium complies with the requirements of the ISO Standard 16654 for the detection of Escherichia coli (E. coli) serotype 0157:H7 in foods as well as with the methods of FDABAM for the isolation of enterohemorrhagic E. coli (EHEC). Mode of Action The nutrient substrates contained in mTSB broth provide favourable growth conditions. The mixture of bile salts No. 3 and Novobiocin suppresses the growth of the Gram-positive microbial flora. Typical Composition (g/litre) Peptone from caseine 17.0; peptone from soymeal 3.0; sodium chloride 5.0; bile salts No. 3 1.5; D(+)-glucose 2.5; di-potassium hydrogen phosphate 4.0; novobiocin 0.02. Preparation Suspend 33 g in 1 litre of demin. water; autoclave (15 min at 121°C). pH: 7.3 ± 0.2 at 25 °C. The prepared broth is clear and yellowish. The broth is stable for up to 6 month when stored at +2 - +8 °C.