M BROTH (MANNOSE) 500GR MERCK

M BROTH (MANNOSE) MERCK RENDIMENTO 36,2g/l EMBALAGEM 500GR For the accelerated detection of Salmonella in dried foods and feeds within the enrichment serology (ES) procedure. Mode of Action M-(Mannose) Broth is based on the formulation of SPERBER and DEIBEL (1969), eliminating dextrose of APT-Broth to allow citrate to serve as an energy source. Mannose was added to the medium to prevent fibrial agglutination in Salmonella in the serological procedure. Inorganic salt ions stimulate Salmonella growth. Tween® is a source for fatty acids. Typical Composition (g/litre) Yeast extract 5.0; peptone from casein 12.5; D-mannose 2.0; sodium citrate 5.0; sodium chloride 5.0; di-potassium hydrogen phosphate 5.0; manganese chloride 0.14; mangesium sulfate 0.8; ferrous(II) sulfate 0.04; Tween® 80 0.75. Preparation Suspend 36.2 g in 1 litre of demin. water and autoclave (15min at 121 °C). pH: 7.0 ± 0.2 at 25 °C. The prepared broth is clear and yellowish-brown