LISTERIA ENRICHMENT BROTH (LEB) SEG. FDA/IDF-FIL MERCK EMB 500GR

LISTERIA ENRICHMENT BROTH (LEB) BASE SEG FDA/IDF-FIL MERCK RENDIMENTO 36,0g/l EMBALAGEM 500GR Mode of Action The Enrichment Broth is based on the formulation of Casein-peptone Soymeal-peptone Broth (k CASO Broth) with additional 6 g/litre yeast extract. The inhibition of the common bacteria is achieved by the addition of selective supplemnts, e.g. Listeria Selective Supplement acc. to IDF-FIL (Cat. No. 1.11781.0001) or Listeria Selective Supplement acc. to FDA (Cat. No. 1.11883.0001). Typical Composition (g/litre) Peptone from casein 17.0; peptone from soymeal 3.0; D(+)glucose 2.5; sodium chloride 5.0; potassium dihydrogen phosphate 2.5 (CASO-broth); yeast extract 6.0. Preparation Suspend 18 g in 500 ml of distilled water, autoclave (15 min at 121 °C). Dissolve the lyophilisate of 1 vial Listeria-Enrichment-Supplement (Cat. No. 1.11781.0001 or 1.11883.0001) by adding of 1 ml sterile distilled water. Mix gently and add the contents to the Enrichment Broth cooled to under 50 °C. pH: 7.3 ± 0.2 at 25 °C. The prepared broth is clear and brown.