LETHEEN BROTH BASE MODIFICADO 500GR MERCK

LETHEEN BROTH BASE MODIFICADO GRANULADO MERCK RENDIMENTO 37,8g/l EMBALAGEM 500GR Letheen Broth is special formulations for the determination of bacterial activity of quaternary ammonium compounds. The media formulations comply with the recommendations of FDA/BAM (1995). Mode of Action Letheen media are highly nutritious containing Lecithin and Tween® 80 for neutralizing quaternary ammonium compounds. These media are modifications of the AOAC formulae. Letheen Broth Base is used for determining the phenol coefficient of quaternary compounds. Typical Composition (g/litre) Letheen Broth Base: Peptone from meat 20.0; peptone from casein 5.0; meat extract 5.0; yeast extract 2.0; sodium chloride 5.0; lecithin 0.7; sodium bisulfite 0.1. Preparation of Letheen Broth Suspend 37.8 g and 5 ml of Tween® 80 in 1 litre of distilled or demin. water until evenly dispersed. Heat, if necessary, with repeated stirring to dissolve completely and autoclave at 121 °C for 15 min. pH: 7.2 ± 0.2 at 25 °C. The prepared broth is turbid and yellowish-brown. Incubation: 24 - 48 hours at 35 °C aerobically.