LB AGAR (MILLER) MERCK - EMB 500G

LB AGAR (MILLER) MERCK REND. 37g/LITRO EMBALAGEM 500G Mode of Action LB-Agar is based on the formulation of MILLER (1972) supporting growth of E. coli. Casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins. Typical Composition (g/litre) LB-Agar: Yeast extract 5.0; peptone from casein 10.0; sodium chloride 10.0; agar-agar 12.0. Preparation Suspend 37 g LB-Agar in 1 litre of demin. water and autoclave for 15 min at 121 °C. pH: 7.0 ± 0.2 at 25 °C. The prepared agar is clear and yellowish-brown.