KANAMYCIN ESCULIN AZIDE AGAR MERCK - EMB 500G

KANAMYCIN ESCULIN AZIDE AGAR MERCK RENDIMENTO 47,5g/l Embalagem 500g. Mode of Action Kanamycin and azide largely inhibit the accompanying bacterial flora. D-streptococci are, however, only slightly sensitive to these substances, so they can grow almost normal and hydrolyse the glucoside esculin to give glucose and esculetin. Esculetin forms an olive green to black complex with iron(III) ions. Typical Composition (g/litre) Peptones from casein 20.0; yeast extract 5.0; sodium chloride 5.0; sodium citrate 1.0; sodium azide 0.15; kanamycin sulfate 002; esculin 1.0; ammonium iron(III) citrate 0.5; agar-agar 15.0. Preparation Suspend 47.5 g/litre, autoclave (15 min at 121 °C), and pour plates. Do not overheat. pH: 7.1 ± 0.2 at 25 °C. The plates are clear and brown-bluish.