FRASER LISTERIA SELECTIVE ENRICHMENT BROTH 500GR MERCK

FRASER LISTERIA SELECTIVE ENRICHMENT BROTH (BASE) MERCK RENDIMENTO 55,0g/l EMBALAGEM 500GR Typical Composition (g/litre) Proteose peptone 5.0; peptone from casein 5.0; yeast extract 5.0; meat extract 5.0; sodium chloride 20.0; disodium hydrogen phosphate 9.6; potassium dihydrogen phosphate 1.35; esculin 1.0; lithium chloride 3.0. Preparation Suspend 55.0 g in 1 litre demin. water and autoclave (15 min at 121 °C). To prepare half-concentraded FRASER broth, dissolve the contents of 1 vial amonium iron(III) citrate and 1 vial of selective supplement (Cat. No. 1.10399.0001 FRASER Supplement) in 1 ml of sterile distilled water each and add to the broth after it has cooled below 50 °C. FRASER broth is made by adding a further bottle of selective supplement to the half- concentrated FRASER broth. The supplements are homogeneously distributed in the broth by carefully swirling. pH: 7.2 ± 0.2 at 25 °C. The prepared broth is clear to almost clear and yellowish-brown.