DICHLORAN GLYCEROL (DG 18) AGAR MERCK - EMB 500G

DICHLORAN GLYCEROL (DG 18) AGAR MERCK RENDIMENTO 31,6g/l Embalaem 500g. Mode of Action By reducing the water activity from approx. 0.99 to 0.95 with 18 % (w/w) glycerol and addition of chloramphenicol growth of bacteria is prevented. The inclusion of dichloran serves to inhibit the rapid spreading of mucoraceous fungi and restricts colony sizes of other genera, easing the colony count. Typical Composition (g/litre) Peptone 5.0; glucose 10.0; potassium dihydrogen phosphate 1.0; dichloran 0.002; magnesium sulfate 0.5; chloramphenicol 0.1; agar-agar 15.0. pH:5.6 ± 0.2 at 25 °C. The prepared plates are clear and yellowish. Preparation Suspend 31.6 g in 1 litre of demin water and heat to boiling until completely dissolved. Add 175 ml of glycerol p.a. (Merck Cat. No.1.04092.) to the medium, mix and autoclave at 121 °C for 15min. Cool to approx. 50 °C, mix well and pour plates.