CHROMOCULT ENTEROCOCCI AGAR MERCK - EMB 500G

CHROMOCULT ENTEROCOCCI AGAR MERCK RENDIMENTO 33,0g/l Embalagem 500g. Mode of Action The presence of Enterococci, especially E. faecalis, E. faecium, E. durans and E. hirae, serves as an indicator for faecal contamination. Growth of Enterococci is stimulated by selected peptones, phosphates and addition of Tween® 80. Enterococci cleave the unique chromogenic substrates in the medium. This produces red colonies allowing an easy detection of Enterococci. Sodium azide and ox bile inhibit most accompanying microbial flora. Non-Enterococci produce colourless, blue/violet or turquoise colonies. These colonies are easily distinguished from the red coloured colonies Enterococci produce. Typical Composition (g/litre) Peptones 10.0; sodium chloride 5.0; sodium azide 0.2; di-potassium hydrogenphosphate 3.4; potassium di-hydrogen-phosphate 1.6; ox bile 0.5; Tween® 80 1.0; chromogenic-mixture 0.25; agar-agar 11.0. Preparation Suspend 33.0 g in 1 litre of demin. water by heating in a boiling water bath or in a flowing steam. Stir the contents to assist dissolution (approx. 45 minutes), let the medium cool to 45-50 °C and pour into plates. ! Do not autoclave! Do not overheat! pH. 7.0 ± 0.2 at 25 °C The plates are clear and slightly yellow. If stored at +4 ± 2 °C and protected from light the plates are stable for 2 weeks. Evaluation Enterococci: Red colonies with a diameter of 0.5 to 2 mm Non-Enterococci: colourless (e.g. Aerococcus viridans ATCC 29503) blue/violet (e.g. Aerococcus viridans ATCC 10400) turquoise (e.g. Streptococcus equi ATCC 33398)