CALDO M EC COM NOVOBIOCINA MERCK (EMB. 500 GR)

M EC BROTH COM NOVOBIOCINA MERCK RENDIMENTO 36,7g/l P/E.COLI 0157:H7 EMBALAGEM 500GR For the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods. The culture medium complies with the requirements of the USDA-FSIS method for the isolation and identification of Escherichia coli 0157:H7 from meat. Mode of Action The nutrient substrates contained in mEC Broth provide favourable growth conditions. Especially lactose improves proliferation of lactose-positive bacteria. The mixture of bile salts No. 3 and Novobiocin suppresses the growth of Grampositive microbial flora. Typical Composition (g/litre) Peptone 20.0; sodium chloride 5.0; bile salts No. 3 1.12; lactose 5.0; di-potassium hydrogen phosphate 4.0; potassium dihydrogen phosphate 1.5; novobiocin 0.02. Preparation Suspend 36.7 g in 1 litre of demin. water; autoclave (15 min at 121 °C). pH: 6.9 ± 0.2 at 25 °C. The prepared broth is clear and yellow-orange. The broth is stable for up to 6 months when stored at +2 to +8 °C. Experimental Procedure and Evaluation Inoculate mEC Broth with the sample material (usually add 25 g test portion of the sample to 225 ml of broth). Incubation: 18 to 24 h at 37 °C or 41.5 °C. Incubation temperature depends on standard used. Afterwards about 0.1 ml of the broth is streaked on the dry surface of a E. coli 0157:H7 Selective Agar, e.g. Fluorocult® E. coli 0157:H7 Agar, SMAC Agar or CT-SMAC Agar in a way that single colonies can be well isolated.