CALDO LAURIL SULFATO C/ FLUORESCENCIA MUG FLUOROCULT MERCK - 500GR

FLUOROCULT LAURYL SULFATE BROTH (LST MUG MEDIUM) MERCK P/ MICROBIOLOGIA RENDIMENTO 36,5g/l (CALDO LAURIL SULFATO C/MUG) EMBALAGEM 500GR The medium complies with the German-DIN-Norm 10183 for the examination of milk, with the regulations acc. to par.35 LMBG (01.00/54) for the examination of food, and acc. to ISO/DIS 11886 - 2.2 (1994) for milk and milk products. Furthermore to the German Badegewässerverordnung (regulations for bathing water) 76/1604 EWG (1995). Mode of Action The lauryl sulfate largely inhibits the growth of undesirable microbial flora. The presence of E. coli is indicated by fluorescence under a long wavelength UV lamp. A positive indole reaction and gas formation due to fermentation of lactose confirm the results. SCHINDLER (1991) recommended the use of this medium in the quality control of bathing water. Typical Composition (g/litre) Tryptose 20.0; lactose 5.0; sodium chloride 5.0; sodium lauryl sulfate 0.1; di-potassium hydrogen phosphate 2.75; potassuim dihydrogen phosphate 2.75; L-tryptophan 1.0; 4-methylumbelliferyl-b-D-glucuronide 0.1. Preparation Suspend 36.5 g/litre, fill in test tubes fitted with DURHAM tubes; autoclave (15 min at 121 °C). pH: 6.8 ± 0.2 at 25 °C. The prepared broth is clear and yellowish-brown.