CALDO DE ENRIQUECIMENTO SELETIVO BOLTON MERCK - EMB 500G

BOLTON SELECTIVE ENRICHMENT BROTH BASE MERCK RENDIMENTO 27,6g/l MEIO ENRIQ SELETIVO P/CAMPYLOBACTER Embalagem 500g. Mode of Action Bolton Selective Enrichment Broth contains nutrients to aid resuscitation of sublethally damaged cells of Campylobacter. By this microaerophilic incubation is not needed. Addition of Bolton Broth Selective Supplement inhibits the accompanying grampositive and gramnegative bacteria as well as yeasts and moulds. Typical Composition (g/litre) Peptone from Meat 10,0; Lactalbumin hydrolysate 5,0; Yeast Extract 5,0; Sodium chloride 5,0; a-ketoglutaric acid 1,0; Sodium pyruvate 0,5; Sodium metabisulphite 0,5; Sodium carbonate 0,6; Haemin 0,01 Preparation Dissolve 13,8 g in 500 ml of demin water. Autoclave (15 min. at 121°C). Cool to 45 - 50°C. Aseptically add 25 ml lysed horse blood and the content of 1 vial of Bolton Broth Selective Supplement. Mix well and distribute the broth into sterile screw top containers. After the adition of the sample the space between screw top and broth should be approx. 2cm. pH: 7.4 ± 0.2 at 25 °C. The ready-to-use broth in the container is dark red to black.