CALDO CAYE MOD. SEG. EVANS MERCK - EMB 100G

CAYE BROTH MODIFIED SEG EVANS MERCK RENDIMENTO 7,95g/200ml Embalagem 100g. Medium for the culture of pathogenic E.coli to enhance verotoxin production during growth. Mode of Action Casaminoacids and yeast extract promote the growth of verotoxin producing E.coli. Trace elements and the high pH-value additionally support the formation of verotoxins. Typical Composition (g/litre) Casaminoacids 20.0; yeast extract 6.0; D(+)glucose 2.5; sodium chloride 2.5; di-potassium hydrogen phosphate 8.71; magnesium sulphate 0.05; manganese chloride 0.005. Preparation Suspend 7,95 g in 200 ml demin. water and heat in a boiling water bath or in a steam jet, swirling regularly, until the medium has completely dissolved. Autoclave (15 min. at 121 °C). Cool the medium down to room temperature. Add aseptically one vial of the prepared CAYE broth Supplement to the CAYE broth; mix. Fill 1 ml under agitation into sterile tubes. pH: 8.5 ± 0.2 at 25 °C. The prepared broth is light brown and clear. Occuring precipitates do not have any effect to the properties of the medium. NECESSÁRIO SUPLEMENTO CÓD. 1000510010.