BUFFERED LISTERIA ENRICHMENT BROTH BASE ACC TO FDA/BAM RENDIMENTO 48,0g/l MERCK EMB 500GR

BUFFERED LISTERIA ENRICHMENT BROTH BASE ACC TO FDA/BAM MERCK RENDIMENTO 48,0g/l EMBALAGEM 500G For the selective enrichment of Listeria spp. Mode of Action The enrichment broth is a modification of the formulation of Tryptic Soy Broth (CASO) with the addition of 6 g/l yeast extract and by increasing its buffering strength. Dextrose is the carbohydrate source. Sodium chloride maintains the osmotic balance of the medium. Phosphate acts as a buffer. Sodium pyruvate mediates the recovery of sublethally damaged Listeria spp. The addition of acriflavine, cycloheximide and nalidixic acid inhibits the growth of the accompanying flora. Typical Composition (g/litre) Tryptic Soy Broth 30 g; yeast extract 6.0; di-sodium hydrogen phosphate 9.6; potassium dihydrogen phosphate 1.35; sodium pyruvate 1.1. Preparation Suspend 24 g in 500 ml of demin. water, dissolve and dispense 225 ml aliquots. Autoclave (15 min at 121 °C). pH: 7.3 ± 0.2 at 25 °C. The prepared broth is clear.