ANAEROBIC AGAR SEG. BREWER MERCK - EMB 500G

ANAEROBIC AGAR SEG BREWER MERCK RENDIMENTO 51,0g/l Embalagem 500g Mode of Action The medium contains a series of reducing agents (thioglycollate, formaldehydesulfoxylate, cystine) which ensure adequate anaerobiosis (QUASTEL and STEPHENSON 1926, AUBERTIN etal. 1928). Methylene blue serves as a redox indicator, its decolouration indicates anaerobiosis. Typical Composition (g/litre) Peptone from casein 10.0; peptone from soymeal 5.0; yeast extract 5.0; L-cystine 0.4; D(+)glucose 10.0; sodium chloride 5.0; sodium thioglycollate 2.0; sodium formaldehydesulfoxylate 1.0; methylene blue 0.002; agar-agar 12.6. Preparation and Storage Usable up to the expiry date when stored dry and tightly closed at +15 to +25 °C. Protect from light.After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25°C. Suspend 51g/litre, autoclave (15 min at 121°C), pour plates to give thick layers. pH: 7.2 ± 0.2 at 25 °C. The plates are clear and light green.