AGAR SPS (PERFRINGES SELECTIVE AGAR SEG ANGELOTTI) MERCK - EMB./500 GR

SPS AGAR (PERFRINGES SELECTIVE AGAR SEG ANGELOTTI) MERCK RENDIMENTO 40,0g/l GRANULADO EMBALAGEM 500G Mode of Action Sulfite Polymyxin Sulfadiazine Agar contains a broad spectrum of nutrients. Sulfite is reduced by most clostridia (including Cl. perfringens) to sulfide, which reacts with iron citrate and causes the colonies to turn black. Other sulfite-reducing microorganisms are largely suppressed by polymyxin and sulfadiazine (sulfapyrimidine). The low sulfite content allows growth of even sulfite-sensitive clostridia which also exhibit an adequate blackening of the colonies (PUT et al. 1961; BEERNS et al. 1961). Typical Composition (g/litre) Peptone from casein 15.0; yeast extract 10.0; iron(III) citrate 0.5; sodium sulfite 0.5; Polymyxin B sulfate 0.01; sodium sulfadiazine 0.12; agar-agar 13.9. Preparation Suspend 40 g/litre, autoclave (15 min at 121 °C). pH: 7.0 ± 0.2 at 25 °C. The prepared medium is clear and yellowish-brown.