AGAR SELETIVO ENTEROCOCCUS SEG. SLANETZ MERCK - EMB 500G

MEMBRANE FILTER ENTEROCOCCUS SELECTIVE AGAR BASE MERCK RENDIMENTO 41,5g/l Embalagem 500g. Mode of Action The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce TTC to give a red formazan, their colonies are thus red in colour. According to LACHICA and HARTMAN (1968), the selectivity for enterococci can be improved by adding carbonate and Tween80®. Typical Composition (g/litre) Tryptose 20.0; yeast extract 5.0; D(+)glucose 2.0; di-potassium hydrogen phosphate 4.0; sodium azide 0.4; agar-agar 10.0. Preparation Suspend 41.5 g/litre, sterilize by heating for 20 minutes in a current of steam (e.g. autoclave without excess pressure). Afterwards cool rapidly! Do not autoclave. Add 10 ml of a filtersterilized 1% 2,3,5-triphenyltetrazolium chloride solution/litre to the base medium at a temperature of approximately 50 °C. Pour plates. pH: 7.2 ± 0.2 at 25 °C. The plates are clear and yellowish-brown.