AGAR BISMUTH SULFITE GRANULADO MERCK - EMB 500G

BISMUTH SULFITE AGAR GRANULADO MERCK MS 80141300389 RENDIMENTO 47,5g/l (SEG. WILSON-BLAIR) SHELF LIFE 1 ANO Embalagem 500g Mode of Action Brilliant green and bismuth largely inhibit the accompanying bacterial flora. Colonies of H2-S-positive salmonellae exhibit blackening due to the formation of iron sulfide. Reduction of bismuth ions to metallic bismuth produces a metallic lustre around the colonies (McCOY 1962). Typical Composition (g/litre) Meat extract 5.0; peptone from meat 10.0; D(+)glucose 5.0; disodium hydrogen phosphate 4.0; iron(III) sulfate 0.3; brilliant green 0.025; bismuth sulfite indicator 8.0; agar-agar 15.0. Preparation and Storage Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light.After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25°C. Suspend 47.5 g/litre, mix the resulting precipitate to give a uniform suspension, pour plates to give thick layers (25 ml). Do not autoclave. pH: 7.6 ± 0.2 at 25 °C. The prepared medium is turbid and green in colour.