MULLER KAUFFMANN TETRATHIONATE NOVOBIOCINE BROTH MERCK - 500g
Modelo: 1058780500
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Descrição
MULLER KAUFFMANN TETRATHIONATE NOVOBIOCINE BROTH SEG. ISO 6579 C/NOVOBIOCINA MERCK
RENDIMENTO 89,5g/l
Embalagem 500g.
For the selective enrichment of salmonellae from food and animal feed stuffs acc. to ISO 6579.
Mode of Action
Tetrathionate is produced from thiosulfate by adding iodine to the culture medium. Tetrathionate suppresses the growth of coliform and other enteric bacteria. Salmonella, Proteus and some other speices of bacteria can reduce tetrathionate and are not inhibited. Calcium carbonate buffers the sulphuric acid, which is liberated when tetrathionate is reduced. Bile promotes the growth of Salmonella, but largely inhibits the accompanying
bacteria. Brillant green and novobiocin suppresses primarily Gram-positive bacteria.
Typical Composition (g/litre)
Meat extract 4.3; peptone from casein 8.6; sodium chloride 2.6; calcium carbonate 38.7; sodium thiosulfate water free 30.5 (equivalent to 47,8 g sodium thiosulphate pentahydrate); ox bile 4.78, brillant green 0.0096; novobiocin 0.040.
Also to be added: Potassium iodide 5.0; iodine 4.0; dissolved in 20 ml water.
Preparation
Suspend 89.5 g in 1000 ml demin. water, heat briefly (5 min.) by boiling and cool rapidly. A sediment of calcium carbonate appears in the turbid broth at the bottom of the tubes. Adjust the pH, if necessary, so that it is 8.0 ± 0.2 at 25 °C.
Do not autoclave.
Prior to use add 20 ml iodine/potassium iodide solution to 1000ml of basal medium. Dispense the medium aseptically into sterile flasks of suitable capacity to obtain the portions necessary for the test. Avoid further heating.
The basal medium without iodine/potassium iodide solution has at refrigeration a shelf life of up to 4 weeks at
2-8°C
Preparation ot the iodine/potassium iodide solution:
Completely dissolve 5 g potassium iodide in 2 ml of water, then add 4 g iodine and dilute to 20 ml distilled water.
n The ready-to-use broth prepared and used the same day. The medium is turbid and green with a white sediment (calcium carbonate).
RENDIMENTO 89,5g/l
Embalagem 500g.
For the selective enrichment of salmonellae from food and animal feed stuffs acc. to ISO 6579.
Mode of Action
Tetrathionate is produced from thiosulfate by adding iodine to the culture medium. Tetrathionate suppresses the growth of coliform and other enteric bacteria. Salmonella, Proteus and some other speices of bacteria can reduce tetrathionate and are not inhibited. Calcium carbonate buffers the sulphuric acid, which is liberated when tetrathionate is reduced. Bile promotes the growth of Salmonella, but largely inhibits the accompanying
bacteria. Brillant green and novobiocin suppresses primarily Gram-positive bacteria.
Typical Composition (g/litre)
Meat extract 4.3; peptone from casein 8.6; sodium chloride 2.6; calcium carbonate 38.7; sodium thiosulfate water free 30.5 (equivalent to 47,8 g sodium thiosulphate pentahydrate); ox bile 4.78, brillant green 0.0096; novobiocin 0.040.
Also to be added: Potassium iodide 5.0; iodine 4.0; dissolved in 20 ml water.
Preparation
Suspend 89.5 g in 1000 ml demin. water, heat briefly (5 min.) by boiling and cool rapidly. A sediment of calcium carbonate appears in the turbid broth at the bottom of the tubes. Adjust the pH, if necessary, so that it is 8.0 ± 0.2 at 25 °C.
Do not autoclave.
Prior to use add 20 ml iodine/potassium iodide solution to 1000ml of basal medium. Dispense the medium aseptically into sterile flasks of suitable capacity to obtain the portions necessary for the test. Avoid further heating.
The basal medium without iodine/potassium iodide solution has at refrigeration a shelf life of up to 4 weeks at
2-8°C
Preparation ot the iodine/potassium iodide solution:
Completely dissolve 5 g potassium iodide in 2 ml of water, then add 4 g iodine and dilute to 20 ml distilled water.
n The ready-to-use broth prepared and used the same day. The medium is turbid and green with a white sediment (calcium carbonate).
Etiquetas: muller kauffmann, novobiocine
