PSEUDOMONAS SELECTIVE AGAR BASE MERCK
RENDIMENTO 48,4g/l
EMBALAGEM 500G

Pseudomonas CFC Selective Agar
When supplemented with Pseudomonas CFC Selective Supplement (Cat.No.1.07627.) the medium complies with the recommendations of ISO 13720 for the detection and enumeration of Pseudomonas spp. in foods and animal feed.

Pseudomonas CN Selective Agar
When supplemented with Pseudomonas CN Selective Supplement (Cat.No. 1.07624) the medium complies with the ecommendations of DIN/EN 12780 for the detection and enumeration of Pseudomonas aeruginosa in water using the membrane filtration technique.

Mode of Action
The peptone mixture in Pseudomonas Selective Agar Base allows growth of a broadspectrum of Pseudomo- nades. The amount of potassium sulfate and magnesium chloride supports forming of pigments.
By use of the appropriate selective supplement and the incubation temperature the medium becomes selective for Pseudomonas spp. including Burkholderia cepacia, formerly known as Pseudomonas cepacia (CFC Agar), or Pseudomonas aeruginosa (CN Agar).

Typical Composition (g/Liter)
Peptone from gelatine 16.0;
Casein hydrolysate 10.0;
potassium sulfate 10.0;
magnesium chloride 1.4;
agar-agar 11.0.

Preparation
Suspend 24.2 g in 500 ml of purified water, add 5 ml glycerol and heat to boiling until dissolved completely.
Autoclave for 15 min. at 121°C.Cool the medium to 45- 50°C and aseptically add the contents of one vial of Pseudomonas CFC Selective Supplement (Cat.No.1.07627) or Pseudomonas CN Selective Supplement
(Cat.No.1.07624). Mix thoroughly and pour plates.
pH: 7.1 ± 0.2 at 25 °C.
The prepared plates are clear and colorless and can be stored for up to 4 weeks at 2 - 8°C in the refrigerator.
Protect from light and drying. Do not keep the liquid medium (45 - 50°C) longer than 4 hours. Do not remelt the medium several times.

Experimental Procedure and Evaluation
Pseudomonas CFC Selective Agar
Inoculate the medium using the surface spread method. Incubation: 44 ± 4 hours at 25 ± 1°C. All grown colonies are suspect Pseudomonas spp. and counted as such.
The suspect colonies must be confirmed. Colonies which show a positive oxidase reaction but no glucose fermentation are confirmed Pseudomonas spp. colonies.

Pseudomonas CN Selective Agar
Inoculate the medium using the membrane filtration technique. The filter material impacts results. Good results were achieved using Cellulose-Mixed Ester membranes (e.g. Pall GN-6).
Incubation: 44 ± 4 hours at 36 ± 2°C.
Check the membrane filters for growth after 22 ± 2 h and 44 ± 4h.
All grown colonies with a blue-green pigmentation are consid-ered confirmed Pseudomonas aeruginosa colonies and counted as such.
Check the membrane filters under UV-light. All colonies not showing the blue-green pigmentation but fluoresce are suspect P. aeruginosa colonies and confirmed by use of acetamide solution.
All other reddish-brown pigmenting colonies, which do not fluoresce are considered suspect P. aeruginosa colonies and confirmed by the oxidase test, acetamide solution and King's B Medium.