AGAR SELETIVO ENTEROCOCCUS SEG. SLANETZ MERCK - EMB 500G
Modelo: 1052890500
-
R$2.178,51
ou até 3x de R$726,17 sem juros
Economize 5% no PIX: R$2.069,58
ou 3.5% no boleto: R$2.102,26
Descrição
MEMBRANE FILTER ENTEROCOCCUS SELECTIVE AGAR BASE MERCK
RENDIMENTO 41,5g/l
Embalagem 500g.
Mode of Action
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce TTC to give a red formazan, their colonies are thus red in colour. According to LACHICA and HARTMAN (1968), the selectivity for enterococci can be improved by adding carbonate and Tween80®.
Typical Composition (g/litre)
Tryptose 20.0; yeast extract 5.0; D(+)glucose 2.0; di-potassium hydrogen phosphate 4.0; sodium azide 0.4; agar-agar 10.0.
Preparation
Suspend 41.5 g/litre, sterilize by heating for 20 minutes in a current of steam (e.g. autoclave without excess pressure). Afterwards cool rapidly!
Do not autoclave.
Add 10 ml of a filtersterilized 1% 2,3,5-triphenyltetrazolium chloride solution/litre to the base medium at a temperature of approximately 50 °C. Pour plates.
pH: 7.2 ± 0.2 at 25 °C.
The plates are clear and yellowish-brown.
RENDIMENTO 41,5g/l
Embalagem 500g.
Mode of Action
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce TTC to give a red formazan, their colonies are thus red in colour. According to LACHICA and HARTMAN (1968), the selectivity for enterococci can be improved by adding carbonate and Tween80®.
Typical Composition (g/litre)
Tryptose 20.0; yeast extract 5.0; D(+)glucose 2.0; di-potassium hydrogen phosphate 4.0; sodium azide 0.4; agar-agar 10.0.
Preparation
Suspend 41.5 g/litre, sterilize by heating for 20 minutes in a current of steam (e.g. autoclave without excess pressure). Afterwards cool rapidly!
Do not autoclave.
Add 10 ml of a filtersterilized 1% 2,3,5-triphenyltetrazolium chloride solution/litre to the base medium at a temperature of approximately 50 °C. Pour plates.
pH: 7.2 ± 0.2 at 25 °C.
The plates are clear and yellowish-brown.
