AGAR SELETIVO P/ PALCAM SEG. VAN NETTEN MERCK EMB 500GR
Modelo: 1117550500
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Descrição
PALCAM LISTERIA SELECTIVE AGAR BASE SEG VAN NETTEN MERCK
RENDIMENTO 71,8g/l
EMBALAGEM 500GR
Mode of Action
PALCAM Agar provides a quantitative cultivation of Listeria monocytogenes, while, at the same time, inhibiting the Gramnegative and most of the Gram-positive accompanying bacteria. The selectivity of the medium results form its content of polymyxin, acriflavin, ceftazidime and lithium chloride. L.monocytogenes breaks down the esculin in the medium to glucose and esculetin. Esculetin forms an olive-green to black complex with iron(III) ions which stains the colonies of L.monocytogenes. Mannitol-positive accompanying bacteria such as staphylococci grow as yellow colonies, if they are not inhibited.
According to Hammer et al. (1990) PALCAM Agar is superior, with respect to selectivity, compared to other listeria media.
Typical Composition (g/litre)
Peptone 23.0;
yeast extract 3.0;
starch 1.0;
sodium chloride 5.0;
agar-agar 13.0 (= Columbia Agar);
D(-)mannitol 10.0;
ammonium iron(III) citrate 0.5;
esculin 0.8;
glucose 0.5;
lithium chloride 15.0;
phenol red 0.08.
Preparation
Suspend 35.9 g in 500 ml of demin. water, autoclave 15 min at 121 °C). Dissolve the contents of 1 vial of PALCAM Listeria Selective Supplement acc. to VAN NETTEN et al. in 1 ml sterile distilled water and add to the sterile medium cooled to 50 °C. If necessary rinse the vial with 1 ml of sterile distilled water. Mix well and pour plates.
pH: 7.2 ± 0.2 at 25 °C.
The prepared plates (incl. supplement) are clear and dark-red.
RENDIMENTO 71,8g/l
EMBALAGEM 500GR
Mode of Action
PALCAM Agar provides a quantitative cultivation of Listeria monocytogenes, while, at the same time, inhibiting the Gramnegative and most of the Gram-positive accompanying bacteria. The selectivity of the medium results form its content of polymyxin, acriflavin, ceftazidime and lithium chloride. L.monocytogenes breaks down the esculin in the medium to glucose and esculetin. Esculetin forms an olive-green to black complex with iron(III) ions which stains the colonies of L.monocytogenes. Mannitol-positive accompanying bacteria such as staphylococci grow as yellow colonies, if they are not inhibited.
According to Hammer et al. (1990) PALCAM Agar is superior, with respect to selectivity, compared to other listeria media.
Typical Composition (g/litre)
Peptone 23.0;
yeast extract 3.0;
starch 1.0;
sodium chloride 5.0;
agar-agar 13.0 (= Columbia Agar);
D(-)mannitol 10.0;
ammonium iron(III) citrate 0.5;
esculin 0.8;
glucose 0.5;
lithium chloride 15.0;
phenol red 0.08.
Preparation
Suspend 35.9 g in 500 ml of demin. water, autoclave 15 min at 121 °C). Dissolve the contents of 1 vial of PALCAM Listeria Selective Supplement acc. to VAN NETTEN et al. in 1 ml sterile distilled water and add to the sterile medium cooled to 50 °C. If necessary rinse the vial with 1 ml of sterile distilled water. Mix well and pour plates.
pH: 7.2 ± 0.2 at 25 °C.
The prepared plates (incl. supplement) are clear and dark-red.
