LETHEEN BROTH BASE MODIFICADO 500GR MERCK
Modelo: 1104050500
-
R$633,07
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Descrição
LETHEEN BROTH BASE MODIFICADO GRANULADO MERCK
RENDIMENTO 37,8g/l
EMBALAGEM 500GR
Letheen Broth is special formulations for the determination of bacterial activity of quaternary ammonium compounds.
The media formulations comply with the recommendations of FDA/BAM (1995).
Mode of Action
Letheen media are highly nutritious containing Lecithin and Tween® 80 for neutralizing quaternary ammonium compounds. These media are modifications of the AOAC formulae. Letheen Broth Base is used for determining the phenol coefficient of quaternary compounds.
Typical Composition (g/litre)
Letheen Broth Base:
Peptone from meat 20.0; peptone from casein 5.0; meat extract 5.0; yeast extract 2.0; sodium chloride 5.0; lecithin 0.7; sodium bisulfite 0.1.
Preparation of Letheen Broth
Suspend 37.8 g and 5 ml of Tween® 80 in 1 litre of distilled or demin. water until evenly dispersed. Heat, if necessary, with repeated stirring to dissolve completely and autoclave at 121 °C for 15 min.
pH: 7.2 ± 0.2 at 25 °C.
The prepared broth is turbid and yellowish-brown.
Incubation: 24 - 48 hours at 35 °C aerobically.
RENDIMENTO 37,8g/l
EMBALAGEM 500GR
Letheen Broth is special formulations for the determination of bacterial activity of quaternary ammonium compounds.
The media formulations comply with the recommendations of FDA/BAM (1995).
Mode of Action
Letheen media are highly nutritious containing Lecithin and Tween® 80 for neutralizing quaternary ammonium compounds. These media are modifications of the AOAC formulae. Letheen Broth Base is used for determining the phenol coefficient of quaternary compounds.
Typical Composition (g/litre)
Letheen Broth Base:
Peptone from meat 20.0; peptone from casein 5.0; meat extract 5.0; yeast extract 2.0; sodium chloride 5.0; lecithin 0.7; sodium bisulfite 0.1.
Preparation of Letheen Broth
Suspend 37.8 g and 5 ml of Tween® 80 in 1 litre of distilled or demin. water until evenly dispersed. Heat, if necessary, with repeated stirring to dissolve completely and autoclave at 121 °C for 15 min.
pH: 7.2 ± 0.2 at 25 °C.
The prepared broth is turbid and yellowish-brown.
Incubation: 24 - 48 hours at 35 °C aerobically.
